Transgenic animal technology : a laboratory handbook
Başlık:
Transgenic animal technology : a laboratory handbook
ISBN:
9780125571661
Basım Bilgisi:
3.bs
Yayım Bilgisi:
Amsterdam ; Boston : Elsevier , c2002.
Fiziksel Açıklamalar:
xv, 696 s. : res. ; 24 cm.
Genel Not:
Kaynakça var.
ection One Overview. Introduction to transgenic animals. Section Two Transgenic animal production focusing on the mouse model. Transgenic animal production using DNA microinjection. Factors affecting transgenic animal production. Gene targeting in embryonic stem cells I: History and methodology. Gene targeting in embryonic stem cells II. Conditional technologies. Retrovirus-mediated gene transfer. Nuclear transfer technologies. Section Three Production of transgenic laboratory and domestic animal species. Production of transgenic rats. Production of transgenic rabbits. Production of transgenic fish. Production of transgenic poultry. Production of transgenic swine by DNA Microinjection. Production of transgenic ruminants by DNA Microinjection. Production of transgenic non-human primates. Retrovirus-mediated gene transfer into oocytes. Section Four: Molecular biology, analyses and enabling technologies Vector design for Transgene Expression. Analysis of transgene integration. PCR optimization for Detection of Transgenic Integration. Evaluation of Transgene Expression. Control of gene expression (from standard gain/loss of function to anti-sense to PNAs). Enabling technologies:Cryopreservation of gametes. Enabling technologies:Embryo culture considerations. Databases, Internet Resources and Genetic Nomenclature.
Özet:
ection One Overview. Introduction to transgenic animals. Section Two Transgenic animal production focusing on the mouse model. Transgenic animal production using DNA microinjection. Factors affecting transgenic animal production. Gene targeting in embryonic stem cells I: History and methodology. Gene targeting in embryonic stem cells II. Conditional technologies. Retrovirus-mediated gene transfer. Nuclear transfer technologies. Section Three Production of transgenic laboratory and domestic animal species. Production of transgenic rats. Production of transgenic rabbits. Production of transgenic fish. Production of transgenic poultry. Production of transgenic swine by DNA Microinjection. Production of transgenic ruminants by DNA Microinjection. Production of transgenic non-human primates. Retrovirus-mediated gene transfer into oocytes. Section Four: Molecular biology, analyses and enabling technologies Vector design for Transgene Expression. Analysis of transgene integration. PCR optimization for Detection of Transgenic Integration. Evaluation of Transgene Expression. Control of gene expression (from standard gain/loss of function to anti-sense to PNAs). Enabling technologies:Cryopreservation of gametes. Enabling technologies:Embryo culture considerations. Databases, Internet Resources and Genetic Nomenclature.