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Part I: Introduction  1. Current Status of the Polyamine Research Field Anthony E. Pegg and Robert A. Casero, Jr.  Part II: Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels  2. Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics Anthony J. Michael  3. Characterization of Genes for Polyamine Modulon Kazuei Igarashi and Keiko Kashiwagi  4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines Lan Xiao and Jian-Ying Wang  5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui  6. Polyamine Block of Inwardly-Rectifying Potassium Channels Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols  Part III: Transgenic Models for Study of Polyamine Function  7. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism David J. Feith  8. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism    Anne Uimari, Mervi T. Hyvönen, Eija Pirinen, and Leena Alhonen  9. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine Xiaojing Wang and Anthony E. Pegg  Part IV: Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification  10. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr.  11. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis  12. Assay of Deoxyhypusine Synthase Activity Edith C. Wolff, Seung Bum Lee, and Myung Hee Park  13. Assay of Deoxyhypusine Hydroxylase Activity Jong Hwan Park, Edith C. Wolff, and Myung Hee Park  Part V: Regulation of Polyamine Content  14. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips  15. Protocols for Studying Antizyme Expression and Function Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji  16. Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family Chaim Kahana  17. Post-Transcriptional Regulation of Ornithine Decarboxylase Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz  Part VI: Polyamine Transport and Uptake  18. Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae Keiko Kashiwagi and Kazuei Igarashi  19. Genetic and Biochemical Analysis of Protozoal Polyamine Transporters Marie-Pierre Hasne and Buddy Ullman  20. Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake  Johanna Welch, Katrin Svensson, Paulina Kucharzewska, and Mattias Belting  21. Polyamine Transport Systems in Mammalian Cells and Tissues Takeshi Uemura and Eugene W. Gerner  22. Procedures to Evaluate the Importance of Dietary Polyamines Paul Acheampong, Mary J. Macleod, and Heather M. Wallace  Part VII: Polyamines and Disease  23. Determination of N1,N12-Diacetylspermine in Urine: A Novel Tumor Marker Masao Kawakita, Kyoko Hiramatsu, Mari Yanagiya, Yosuke Doi, and Mieko Kosaka  24. Spermidine/spermine-N1-Acetyltransferase in Kidney Ischemia Reperfusion Injury Kamyar Zahedi and Manoocher Soleimani  25. Use of Polyamine Metabolites as Markers for Stroke and Renal Failure Kazuei Igarashi and Keiko Kashiwagi  26. Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection Alain P. Gobert, Rupesh Chaturvedi, and Keith T. Wilson  27. High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantification of Three Major Polyamines in Post-Mortem Brain Cortex            Gary Gang Chen, Laura M. Fiori, Orval A. Mamer, and Gustavo Turecki  28. Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (Snyder-Robinson Syndrome)            Charles E. Schwartz, Xaiojing Wang, Roger E. Stevenson, and Anthony E. Pegg  Part VIII: Chemistry and Analysis  29. Methylated Polyamines as Research Tools            Alex R. Khomutov, Janne Weisell, Maxim A. Khomutov, Nikolay A. Grigorenko, Alina R. Simonian, Merja R. Häkkinen, Tuomo A. Keinänen, Mervi T. Hyvönen, Leena Alhonen, Sergey N. Kochetkov, and Jouko Vepsäläinen  30. Fluorescent Substrates for Polyamine Catabolic Enzymes and Transport            Koichi Takao and Akira Shirahata  31. Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors            Patrick M. Woster  32. Measurement of Polyamine pKa Values            Ian S. Blagbrough, Abdelkader A. Metwally, and Andrew J. Geall  33. Polyamine Analysis by LC-MS            Merja R. Häkkinen