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Animal Cell Biotechnology - Methods and Protocols Editor: Ralf Pörtner Contents Preface Contributors Part I. Basic techniques for establishing production cell lines 1 Transduction Technologies David A. Fluri and Martin Fussenegger 2 Cells for gene therapy and vector production C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten 3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier 4 DNA Fingerprinting and Characterisation of Animal Cell Lines Glyn N. Stacey, Ed Byrne and J. Ross Hawkins Part II. Basic cultivation techniques 5 Microcarrier Cell culture Technology Gerald Blüml 6 Cell Encapsulation Holger Hübner 7 Tools for High-Throughput Medium and Process Optimisation Martin Jordan and Nigel Jenkins Part III. Cell characterisation and analysis 8 Cell counting and viability measurements Michael Butler and Maureen Spearman 9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai 10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors André A. Neves and Kevin M. Brindle 11 Methods for off-line analysis of nutrients and products in mammalian cell culture Heino Büntemeyer 12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation Derek Adams, Rashmi Korke, and Wei-Shou Hu 13 Measurement of apoptosis in cell culture Adiba Ishaque1 and Mohamed Al-Rubeai 14 Metabolic Flux Estimation in Mammalian Cell Cultures Chetan T. Goudar, Richard Biener, James M. Piret and Konstantin B. Konstantinov Part IV. Special cultivation techniques 15 Disposable bioreactors for inoculum production and protein expression Regine Eibl and Dieter Eibl 16 Hollow Fibre Cell Culture John M. Davis 17 Cultivation of mammalian cells in fixed bed reactors Ralf Pörtner and Oscar B.J. Platas Barradas 18 Configuration of Bioreactors Dirk E. Martens and Evert Jan van den End Part V. Downstream techniques 19 Membrane filtration in animal cell culture Peter Czermak, Dirk Nehring and Ranil Wickramasinghe 20 Chromatographic techniques in the downstream processing of (recombinant) proteins Ruth Freitag Part VI. Special applications 21 Vaccine production - state of the art and future needs in upstream processing Yvonne Genzel and Udo Reichl 22 Retrovirus production and characterization Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves 23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production Sabine Geisse Index